東京海洋大学 産学・地域連携推進機構 研究者総覧データベース

氏名

高橋肇 

読み

TAKAHASHI,Hajime

役職

教授

TEL・FAX

E−mail

*研究者への技術相談等は、「海の相談室」をご利用ください。

ホームページ

所属部門

食品生産科学部門

担当科目

微生物学　食品微生物学実験　微生物学実験

キーワード

食品衛生、食品微生物、遺伝子検出、DNAタイピング

研究テーマ・活動内容

１）遺伝子手法による食品中の有害微生物の検出・同定に関する研究
２）食品中に存在する有害微生物のDNAタイピング

SDGsと関係する研究テーマ

研究と関係するSDGs項目

自己アピール

技術相談対応分野

共同研究の希望課題

科研費等研究テーマ

平成18年度-19年度　科学研究費補助金(若手B)　
課題番号；18700621 「食品製造現場や環境中における糞便汚染指標大腸菌の迅速定量法」
平成22年度-24年度　科学研究費補助金(若手B)　
課題番号；22780191「分子生物学的手法を用いた腸内細菌科菌群の迅速定量法と同定システムの構築」
平成20年度ー24年度　農林水産技術会議
「生産・流通・加工工程における体系的な危害要因の特性解明とリスク低減技術の開発」
平成22年ー24年　農林水産技術会議　実用開発プロジェクト
「バイオジェニックアミン類蓄積抑制技術の開発による日本産水産物の競争力強化」　

講演テーマ

特記事項

氏名

高橋肇 

読み

TAKAHASHI,Hajime

役職

教授

所属部門

食品生産科学部門

学歴

2003年3月　東京水産大学大学院水産学研究科　博士後期課程学位取得修了

経歴

2003年10月～　社団法人　日本食品衛生協会リサーチレジデント
　　　　　　　国立医薬品食品衛生研究所　衛生微生物部勤務

2005年4月～　　山脇学園短期大学　食物科　専任講師

2007年4月～　　現職

学位

博士（水産学）

学位論文

所属学会と役職など

日本食品衛生学会、日本食品微生物学会、日本感染症学会、日本細菌学会、日本水産学会

社会活動

受賞歴等

氏名

高橋肇 

読み

TAKAHASHI,Hajime

役職

教授

所属部門

食品生産科学部門

主要論文

1. Bon Kimura, Satoshi Hokimoto, Hajime Takahashi and Tateo Fujii.
Photobacterium histaminum Okuzumi et al. 1994 is a later subjective synonym of Photobacterium damselae subsp. damselae (Love et al. 1981) Smith et al. 1991.
International Journal of Systematic and Evolutionary Microbiology (2000), 50; 1339-1342.

2. Hajime Takahashi, Bon Kimura, Mayumi Mori and Tateo Fujii.
Analysis of bacterial communities in Kusaya gravy by denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments.
Japanese Journal of Food Microbiology (2002), 19 (4); 179-185.

3. Hajime Takahashi, Bon Kimura, Miwako Yoshikawa and Tateo Fujii.
Cloning and sequencing of the histidine decarboxylase genes of gram-negative, histamine-producing bacteria and their application in detection and identification of these organisms in fish.
Applied Environmental Microbiology (2003), 69 (5); 2568-2579.

4. Seitaro Goto, Hajime Takahashi, Susumu Kawasaki, Bon Kimura, Tateo Fujii, Miki Nakatuji and Itaru Watanabe.
Detection of Leuconostoc strains at a meat processing plant using polymerase chain reaction.
Journal of Food Hygienic Society of Japan (2004), 45 (1); 25-28.

5. Kunikazu Yamane, Jun Asato, Naofumi Kawade, Hajime Takahashi, Bon Kimura and Yoshichika Arakawa.
Two cases of fatal necrotizing fasciitis caused by Photobacterium damsela in Japan;
Journal of Clinical Microbiology (2004), 42 (3); 1370-1372.

6. Hajime Takahashi, Bon Kimura, Miwako Yoshikawa, Seitaro Gotou, Itaru Watanabe and Tateo Fujii.
Direct detection and identification of lactic acid bacteria in a food processing plant and meat products using denaturing gradient gel electrophoresis;
Journal of Food Protection, (2004), 67 (11); 2515–2520.

7. Hajime Takahashi, Yukiko Hara-Kudo, Jiro Miyasaka, Susumu Kumagai and Hirotaka Konuma.
Development of a quantitative real-time polymerase chain reaction targeted to the toxR for detection of Vibrio vulnificus.
Journal of Microbiological Methods,(2005), 61 (1), 77-85.

8. Hajime Takahashi, Yoshito Iwade, Hirotaka Konuma and Yukiko Hara-Kudo.
Development of a quantitative real-time PCR method for estimation of the total number of Vibrio parahaemolyticus in contaminated seawater and shellfish.
Journal of Food Protection, (2005), 68 (5), 1083-1088.

9. Satoko Handa, Bon Kimura, Hajime Takahashi, Takashi Koda, Kazuo Hisa and Tateo Fujii.
Incidence of Listeria monocytogenes in raw seafood products in Japanese retail stores.
Journal of Food Protection, (2005), 68 (2), 411-415.

10.工藤由起子、三輪憲永、山崎省吾、八柳潤、岩出義人、高橋肇、宮坂次郎
「魚介類からのVibrio vulnificusの定量検出方法の検討」
感染症学雑誌、(2005), 79 (12), 931-936.

11. Hajime Takahashi, Hirotaka Konuma and Yukiko Hara-Kudo.
Development of a quantitative real-time PCR method to enumerate total bacterial counts in ready-to-eat fruits and vegetables. Journal of Food Protection, (2006), 69 (10), 2504-2508.

12. M. Goto, H. Takahashi, Y. Segawa, H. Hayashidani, K. Takatori, Y. Hara-kudo.
Real-time PCR method for quantification of Staphylococcus aureus in milk. Journal of Food Protection, (2007), 70 (1), 90-96.

13. Hajime Takahashi, Miki Sato, Bon Kimura Tatsuya Ishikawa and Tateo Fujii.
Evaluation of the PCR single strand conformational polymorphism analysis for identification gram-negative histamine-producing bacteria isolated from fish. Journal of Food Protection, (2007), 70 (5), 1200-1205.

14. Satoko Handa-Miya, Bon Kimura, Hajime Takahashi, Miki Sato, Tatsuya Ishikawa, Kazunori Igarashi and Tateo Fujii.
Nonsense mutated inlA and prfA not widely distributed in Listeria monocytogenes isolates from ready-to-eat seafood products in Japan. International Journal of Food Microbiology. (2007), 117(3), 312-318.

15. Hajime Takahashi, Satoko Handa-Miya, Bon Kimura, Miki Sato, Asami Yokoi, Seitaro Goto, Itaru Watanabe, Takashi Koda, Kazuo Hisa and Tateo Fujii
Development of multilocus single strand conformation polymorphism (MLSSCP) analysis of virulence genes of Listeria monocytogenes and comparison with existing DNA typing methods. Internationl Journal of Food Microbiology, (2007), 118(3), 274-284.

16. Y. Tanaka, B. Kimura, H. Takahashi, T. Watanabe, H. Obata, A. Kai, S. Morozumi and T. Fujii.
Lysine decarboxylase of Vibrio parahaemolyticus: kinetics of transcription and role in acid resistance. Journal of Applied Microbiology, (2008), 104(5), 1283-1293.

17. Bon Kimura, Yohko Sekine, Hajime Takahashi, Yuichiro Tanaka, Hiromi Obata,Akemi Kai, Satoshi Morozumi, Tateo Fujii.
Multiple-locus variable-number of tandem-repeats analysis distinguishes Vibrio parahaemolyticus pandemic O3:K6 strains. Journal of Microbiological Methods, (2008), 72(3), 313–320.

18. Hajime Takahashi, Bon Kimura, Yuichiro Tanaka, Mayumi Mori, Asami Yokoi and Tateo Fujii.
Use of single-strand conformation polymorphism of amplified16S rDNA for grouping of bacteria isolated from foods. Journal of Food Protection, (2008), 71(4), 839-844.

19. Hajime Takahashi, Satoko Miya, Bon Kimura, Kunikazu Yamane, Yoshichika Arakawa, and Tateo Fujii.
Difference of Genotypic and Phenotypic Characteristics and Pathogenicity Potential of Photobacterium damselae subsp. damselae between Clinical and Environmental Isolates from Japan. Microbial Pathogenesis, (2008), 45(2), 150–158.

20. Satoko Miya, Bon Kimura, Miki Sato, Hajime Takahashi, Tatsuya Ishikawa, Takayuki Suda, Chikako Takakura, Tateo Fujii and Martin Wiedmann.
Development of a multilocus variable-number of tandem repeat typing method for Listeria monocytogenes serotype 4b strains.International Journal of Food Microbiology, (2008), 124(3), 239-249.

21. B. Kimura, H. Takahashi, S. Hokimoto, Y. Tanaka and T. Fujii
Induction of the histidine decarboxylase genes of Photobacterium damselae subsp. damselae (formally P. histaminum) at low pH. Journal of Applied Microbiology (2009), 107(2), 485-497.

22. Hajime Takahashi, Satoko Miya, Kazunori Igarashi, Takayuki Suda, Shintaro Kuramoto and Bon Kimura.
Biofilm Formation Ability of Listeria monocytogenes Isolates from Raw Ready-to-Eat Seafood. Journal of Food Protection (2009), 72(7), 1476-1480.

23 . Hajime Takahashi, Bon Kimura, Yuichiro Tanaka, Junko Shinozaki, Takayuki Suda, and Tateo Fujii. Real-time PCR and enrichment culture for sensitive detection and enumeration of Escherichia coli. Journal of Microbiological Methods (2009), 79(1), 124-127.

24. Takashi Kuda, Reiko Tanibe, Mayumi Mori, Harumi Take, Toshihide Michihata, Toshihiro Yano, Hajime Takahashi, Bon Kimura.
Microbial and chemical properties of aji-no-susu, a traditional fermented fish with rice product in the Noto Peninsula, Japan.
Fisheries Science (2009), 75, 1499–1506.

25. Yuichiro Tanaka, Hajime Takahashi, Nao Kitazawa, and Bon Kimura.
Rapid estimation of microbial populations in fish samples by using terminal restriction fragment length polymorphism analysis of 16S rDNA.
Journal of Food Protection (2010) , 73, 104-113.

26. Yuichiro Tanaka, Hajime Takahashi, Ushio Simidu, and Bon Kimura.
Design of a new universal real-time PCR system targeting the tuf gene for the enumeration of bacterial counts in food
Journal of Food Protection (2010), 73, 670–679.

27. Hajime Takahashi, Chikako Takakura, and Bon Kimura.
A quantitative real-time PCR method for monitoring Clostridium botulinum type A in rice samples.
Journal of Food Protection (2010), 73, 688-694..

28. Yuichiro Tanaka, Hajime Takahashi, Akiya Imai, Tsutomu Asao, Shunji Kozaki, and Bon Kimura.
Reconsideration of flexibility in verifying rapid alternative food microbiological methods.
Food Control (2010), 21, 1075-1079.

29. Satoko Miya, Hajime Takahashi, Tatsuya Ishikawa, Tateo Fujii and Bon Kimura.
Risk of Listeria monocytogenes Contamination of Raw Ready-To-Eat
Seafood Products Available at Retail Outlets in Japan.
Applied and Environmental Microbiology (2010), 76(10), 3383-3386.

30. Hajime Takahashi, Takayuki Suda, Yuichiro Tanaka and Bon Kimura.
Cellular hydrophobicity of Listeria monocytogenes involves initial attachment and biofolm formation on the surface of polyvinyl chloride.
Letters in Applied Microbiology (2010) 50, 618-625.

31. Choa An, Hajime Takahashi, Bon Kimura and Takashi Kuda.
Comparison of PCR-DGGE and PCR-SSCP analysis for bacterial flora of Japanese traditional fermented fish products, aji-narezushi and iwashi-nukazuke.
Journal of the Science of Food and Agriculture (2010) 90,1796–1801.

32. Hajime Takahashi, Shintaro Kuramoto, Satoko Miya, Bon Kimura.
Desiccation survival of Listeria monocytogenes and other potential foodborne pathogens on stainless steel surface is affected by different food soils.
Food Control (2010) 22, 633-637.

33. Takashi Kuda, Taichiro Iwase, Chaturongkasmurit Yuphakhun, Hajime Takahashi, Takashi Koyanagi and Bon Kimura.
Surfactant-disinfectant resistance of Salmonella and Staphylococcus adhered and dried on surfaces with egg compounds.
Food Microbiology (2011) in press

34. Tateo Fujii, Shoko Watanabe, Masako Horikoshi, Hajime Takahashi and Bon Kimura.
PCR-DGGE analysis of bacterial communities in funazushi, fermented crucian carp with rice, during fermentation. Fisheries Science (2011) 77, 151-157.

35. Hajime Takahashi, Shintaro Kuramoto, Satoko Miya, Hiroaki Koiso, Takashi Kuda, and Bon Kimura.
Use of commercially available antimicrobial compounds for prevention of Listeria monocytogenes growth in ready-to-eat minced tuna and salmon roe during shelf life.
Journal of Food Protection (2011) in press

36. Shigenobu Koseki, Yoshiko Takizawa, Satoko Miya, Hajime Takahashi, Bon Kimura. Modeling and predicting the simultaneous growth of Listeria monocytogenes and natural flora in minced tuna. Journal of Food Protection (2011) 74(2):176-187.

36. Hajime Takahashi, Ayumi Ohuchi, Satoko Miya, Yukino Izawa and Bon Kimura.
Effect of food residued on norovirus survival on stainless steel surfaces.
Plos One (2011) 6(8), e21951.

著書

氏名

高橋肇 

読み

TAKAHASHI,Hajime

役職

教授

所属部門

食品生産科学部門

研究室紹介

卒研希望者等へのメッセージ

受験生へのメッセージ