東京海洋大学 海の研究戦略マネジメント機構 研究者総覧データベース

氏名

廣野育生 

読み

HIRONO, Ikuo

役職

教授

TEL・FAX

03-5463-0689

E−mail

hirono@kaiyodai.ac.jp
*研究者への技術相談等は、「海の相談室」をご利用ください。

ホームページ

http://www2.kaiyodai.ac.jp/~h-kondo/genome/index.html

所属部門

海洋生物資源学部門

担当科目

学部
分子生物学、水産遺伝子工学実験
大学院後期課程
ゲノム科学特論、応用生物工学合同セミナー、応用生物工学特別研究
大学院前期課程
遺伝生化学、海洋生物工学特別演習、海洋生物工学特別研究

キーワード

魚介類免疫・生体防御、魚病学、ワクチン、微生物感染症防除、魚介類ゲノム科学

研究テーマ・活動内容

養殖場で発生し経済的に多大な被害を与えている微生物感染症を克服するための研究を行ってます。
ホームページを見て下さい。
http://www2.kaiyodai.ac.jp/~h-kondo/genome/index.html
その他、私に関連するwebsite
research map
https://researchmap.jp/read0187685
J-Global
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901059189172888
Research Gate
https://www.researchgate.net/profile/Ikuo_Hirono

SDGsと関係する研究テーマ

＊世界戦略魚の作出を目指したタイ原産魚介類の家魚化と養魚法の構築
＊クルマエビ類微生物感染症克服のための防除法の開発

研究と関係するSDGs項目

自己アピール

指導学生の学会賞等の受賞等

令和1年度(2019)
　国際マリンバイオテクノロジー会議(2019)学生ポスター賞（川戸智, Ivane P. Gerasmio）
　第12回アジア水産学会大会(2019年)最優秀口頭発表賞(Ivane P. Gerasmio)
平成30年度(2018)
　第20回マリンバイオテクノロジー学会学生ポスター賞（川戸智）
　第6回アジアにおけるケージ養殖国際シンポジウム(2018)最優秀ポスター賞（今泉健太郎）
平成29年度(2017)
　日本水産学会85周年記念国際シンポジウム(2017年)優秀ポスター賞(中村梨夏）
平成28年度
　第18回マリンバイオテクノロジー学会学生ポスター賞（川戸智）
平成24年
　第12回国際比較免疫学会学生ポスター賞（Sheryll Hipolipo)
平成22年度　
　第13回マリンバイオテクノロジー学会学生ポスター賞（設楽愛子）
　第13回マリンバイオテクノロジー学会学生ポスター賞（海津彰弘）
平成21年　
T　he 17th Plant and Animal Genome ConferenceのTravel Awards（小山喬）
第11回国際比較免疫学会学生優秀ポスター賞の2位（Fernand Fagutao）
　平成22年度日本水産学会関東支部会学生ベストポスター賞（鈴木克彰）
平成20年　
　第7回アジア魚病学会ベストポスター賞の1位受賞（Fernand Fagtao）
　第7回アジア魚病学会ベストポスター賞の2位受賞（加藤豪司）
平成17年　
　第6回アジア魚病学会学生最優秀口頭発表賞受賞（Dang Thi Lua）

指導学生が獲得した助成金等
令和2年度笹川科学研究助成金（今泉健太郎）
平成26年度笹川科学研究助成金（王　媛媛）
平成22年度笹川科学研究助成金（加藤豪司）
平成21年度笹川科学研究助成金（設楽愛子）
平成20年度笹川科学研究助成金（小山　喬）

技術相談対応分野

魚介類微生物感染症に関すること。
東南アジア、特にタイで長く共同研究を行っておりますので東南アジアにおけるにおける共同研究についても対応出来ます。
2019~2023年
https://www.kaiyodai.ac.jp/satreps/index.html
2012~2016年
https://www.kaiyodai.ac.jp/exchange/satreps/satreps/index.html
2010~202014年
https://www.kaiyodai.ac.jp/exchange/asia/
2000〜2009年
https://www.kaiyodai.ac.jp/international/network/program.html

共同研究の希望課題

魚類ワクチン、病原微生物診断法、プロバイティクの評価、免疫不活剤の評価
ホームページを見て下さい。

特許 (13件)
海産魚の類結節症に対するDNAワクチン
ヒラメラブドウイルス結合性アプタマー
ウイルス性出血性敗血症ウイルス結合性アプタマー
コイヘルペスウイルス特異的抗体及びその抗原
海産魚のノカルジア症に対するDNAワクチン及びBCGワクチン
コイヘルペスウイルス(KHV)の検出方法
コイヘルペスウイルス(KHV)病用DNAワクチン
海産魚のマダイイリドウイルスに対するDNAワクチン
ヒラメのウイルス性出血性敗血症に対するDNAワクチン
ヒラメラブドウイルス感染魚類用DNAワクチン
ヒラメのリゾチームをコードする遺伝子
パスツレラ・ピシシダの決定遺伝子
エロモナス・サルモニシダの決定遺伝子

科研費等研究テーマ

いろいろ。私の科研費については下記をご覧ください。
https://kaken.nii.ac.jp/ja/search/?kw=%E5%BB%A3%E9%87%8E%E8%82%B2%E7%94%9F

講演テーマ

いろいろ。
興味がある人は連絡下さい。

特記事項

氏名

廣野育生 

読み

HIRONO, Ikuo

役職

教授

所属部門

海洋生物資源学部門

学歴

昭和63年3月宮崎大学農学部水産増殖学科卒業
平成2年3月 宮崎大学大学院農学研究科修士課程修了
平成5年3月 鹿児島大学大学院連合農学研究科博士過程修了

経歴

平成4年4月　日本学術振興会特別研究員DC2
平成6年4月　日本学術振興会特別研究員PD
平成6年11月 東京水産大学大学院資源育成学専攻助手
平成10年1月 文部省在外研究員（若手）にて
スタンフォード大学ホプキンス海洋研究に1年間留学
平成14年7月　東京水産大学大学院資源育成学専攻助教授
平成16年10月　東京海洋大学大学院海洋科学技術研究科准教授（大学統合による）
平成21年3月　同　教授　
平成28年4月　東京海洋大学学術研究院海洋生物資源学部門教授（学内の組織改編による名称変更）

学位

鹿児島大学大学院連合農学研究科　 博士（農学）

学位論文

魚類病原細菌エロモナス属の溶血素遺伝子の構造解析

所属学会と役職など

日本水産学会、日本魚病学会、日本細菌学会、日本生化学会、日本分子生物学会、マリンバイオテクノロジー学会、アジア水産学会、アメリカ微生物学会、国際比較免疫学会の各会員
日本水産学会庶務幹事（平成16年度〜17年度）
日本水産学会総務幹事（平成18年度〜19年度）
日本水産学会編集委員（平成15年度〜20年度）
日本水産学会企画広報委員（平成21年度〜現在）
日本水産学会関東支部評議員（平成19〜現在）
日本魚病学会庶務幹事（平成13〜16年度）
日本魚病学会会長指名幹事（平成17年度〜18年度）
日本魚病学会評議員（平成17年度〜20年度）
日本魚病学会企画幹事（平成21年度〜現在）
日本農学会運営委員（平成21年度日本魚病学会選出）
マリンバイオテクノロジー学会評議員（平成20年度〜現在）
Fish and Shellfish Immunology編集委員(平成17年〜現在)
Fish and Shellfish Immunology編集委員長（2人制）(平成22年〜現在)
Developmental and Comparative Immunology編集委員(平成18年〜23年)
Reviews in Fish Biology and Fisheries編集委員(平成12年〜現在)

社会活動

受賞歴等

平成11年度日本水産学会奨励賞
平成17年度マリンバイオテクノロジー学会論文賞
2005年 Best Paper Published in Marine Biotechnology
平成20年度日本魚病学会奨励賞
平成20年度日本水産学会論文賞
平成21年度日本水産学会論文賞
令和2年度マリンバイオテクノロジー学会賞

氏名

廣野育生 

読み

HIRONO, Ikuo

役職

教授

所属部門

海洋生物資源学部門

主要論文

2020:
1. Pumchan A, Krobthong S, Roytrakul S, Sawatdichaikul O, Kondo H, Hirono I, Areechon N, Unajak S. (2020) Novel chimeric multiepitope vaccine for streptococcosis disease in Nile tilapia (Oreochromis niloticus Linn.). Sci Rep. 10:603. doi: 10.1038/s41598-019-57283-0.
2. Kawato S, Nozaki R, Kondo H, Hirono I. (2020) Draft genome sequences of Vibrio atypicus strains DSM 25292T and TUMSAT1. Microbiol Res Announcements, e01526-19, doi: 10.1128/MRA.01526-19
3. Pedrosa-Gerasmio IR, Kondo H, Hirono I. (2020) Molecular cloning, characterization and gene expression analysis of aminolevulinic acid synthase in Litopenaeus vannamei. Gene, 736: 144421, doi: 10.1016/j.gene.2020.144421
4. Kiataramgul A, Maneenin S, Purton S, Areechon N, Hirono I, Brocklehurst TW, Unajak S. (2020) An oral delivery system for controlling white pot syndrome virus infection in shrimp using transgenic microalgae. Aquaculture, accepted, doi: 10.1016/j.aquaculture.2020.735022
5. Pedrosa-Gerasmio IR, Nozaki R, Kondo H, Hirono I. (2020) Gut bacterial community profile in Pacific white shrimp Litopenaeus vannamei following 5- aminolevulinic acid supplementation. Aquaculture Research, accepted, doi: 10.1111/are.14750
6. Yasumoto K, Koiwai K, Hiraoka K, Hirono I, Kondo H. (2020) Characterization of natural antigen-specific antibodies from naïve sturgeon serum. Developmental and Comparative Immunology, accepted
7. Fauzi IA, Haga Y, Kondo H, Hirono I, Satoh S. (2020) Dietary citrulline improves survival of rainbow trout Oncorhynchus mykiss juveniles challenged with Vibrio anguillarum. Aquaculture, accepted
8. Palang I, Hirono I, SenapinS, Sirimanapong W, Withyachumnarnkul B, Vanichviriyakit R. (2020) Cytotoxicity of Streptococcus agalactiae secretory protein on tilapia cultured cells. J Fish Diseases, accepted, https://doi.org/10.1111/jfd.13230
9. Kondo H, Sano H, Wang Y, Kawase J, Shimanoki E, Jirapongpairoj W, Nozaki R, Hirono I. (2020) Starvation–refeeding causes cellular stress responses in the gut and liver of Masu salmon Oncorhynchus masou masou. Fisheries Science, accepted, doi: 10.1007/s12562-020-01464-8


2019:
1. Kondo H, Chung S, Hirosawa E, Hirono I. (2019) Adjuvant effects on protection and immune response of Japanese flounder immunized by the formalin-killed cells of Edwardsiella tarda. Fish Shellfish Immunol. 84:120-123. doi: 10.1016/j.fsi.2018.09.071.
2. Tinwongger S, Thawonsuwan J, Kondo H, Hirono I. (2019) Identification of an anti-lipopolysaccharide factor AV-R isoform (LvALF AV-R) related to Vp_PirAB-like toxin resistance in Litopenaeus vannamei. Fish Shellfish Immunol. 84:178-188. doi: 10.1016/j.fsi.2018.10.005.
3. Kawato S, Shitara A, Wang Y, Nozaki R, Kondo H, Hirono I. (2019) Crustacean genome exploration reveals the evolutionary origin of white spot syndrome virus. J Virol. pii: JVI.01144-18. doi: 10.1128/JVI.01144-18.
4. Kayansamruaj P, Soontara C, Unajak S, Dong HT, Rodkhum C, Kondo H, Hirono I, Areechon N. (2019) Comparative genomics inferred two distinct populations of piscine pathogenic Streptococcus agalactiae, serotype Ia ST7 and serotype III ST283, in Thailand and Vietnam. Genomics. doi: 10.1016/j.ygeno 2018.11.016.
5. Jariyapong P, Pudgerd A, Cheloh N, Hirono I, Kondo H, Vanichviriyakit R, Weerachatyanukul W, Chotwiwatthanakun C. (2019) Hematopoietic tissue of Macrobrachium rosenbergii plays dual roles as a source of hemocyte hematopoiesis and as a defensive mechanism against Macrobrachium rosenbergii nodavirus infection. Fish Shellfish Immunol. 86:756-763. doi: 10.1016/j.fsi.2018.12.021.
6. Pedrosa-Gerasmio IR, Kondo H, Hirono I. (2019) Dietary 5-aminolevulinic acid enhances ATP production, ecdysis and immune response in Pacific white shrimp, Litopenaeus vannamei (Boone). Aquaculture Research, 50: 1131-1141, doi.org/10.1111/are.13987
7. Fauzi IA, Haga Y, Kondo H, Hirono I, Satoh S. (2019) Effects of arginine supplementation on growth performance and plasma arginine, ornithine and citrulline dynamics of rainbow trout, Oncorhynchus mykiss. Aquaculture Research, 50:1277–1290. Doi: 10.1111/are.14004.
8. Koiwai K, Kondo H, Hirono I. (2019) Isolation and molecular characterization of hemocyte sub-populations in kuruma shrimp Marsupenaeus japonicas. Fisheries Science, 85:521–532. doi.org/10.1007/s12562-019-01311-5
9. Nakamura R, Pedrosa-Gerasmio IR, Alenton RRR, Nozaki R, Kondo H, Hirono I. (2019) Anti-PirA-like toxin immunoglobulin (IgY) in feeds passively immunizes shrimp against acute hepatopancreatic necrosis disease. J Fish Dis. 42:1125-1132 doi: 10.1111/jfd.13024.
10. Alenton RRR, Koiwai K, Nakamura R, Thawonsuwan J, Kondo H, Hirono I. (2019) A Hint of Primitive Mucosal Immunity in Shrimp through Marsupenaeus japonicus Gill C-Type Lectin. J Immunol. 203:2310-2318 pii: ji1900156. doi: 10.4049/jimmunol.1900156.
11. Kawato S, Kobayashi K, Shirakashi S, Yanagi s, Fukuda Y, Yamashita H, Nozaki R, Hirono I, Kondo H. (2019) Phylogenetic Analysis with complete mitochondrial genome sequences of Benedenia seriolae specimens derived from Japanese Seriola spp. Fish Pathology, 54: 27-33.

2018:
9. Koiwai K, Kodera T, Thawonsuwan J, Kawase M, Kondo H, Hirono I. (2018) A rapid method for simultaneously diagnosing four shrimp diseases using PCR-DNA chromatography method. J Fish Dis. 41: 395-399. doi: 10.1111/jfd.12732.
10. Koiwai K, Kondo H, Hirono I. (2018) RNA-seq identifies integrin alpha of kuruma shrimp Marsupenaeus japonicus as a candidate molecular marker for phagocytic hemocytes. Dev Comp Immunol. 81:271-278. doi: 10.1016/j.dci.2017.12.014.
11. Kayansamruaj P, Dong HT, Hirono I, Kondo H, Senapin S, Rodkhum C. (2018) Genome characterization of piscine 'Scale drop and Muscle Necrosis syndrome'-associated strain of Vibrio harveyi focusing on bacterial virulence determinants. J Appl Microbiol. 124:652-666. doi: 10.1111/jam.13676.
12. Imaizumi K, Tinwongger S, Kondo H, Hirono I. (2018) Disinfection of an EMS/AHPND strain of Vibrio parahaemolyticus using ozone nanobubbles. J Fish Dis. 41:395-399. doi: 10.1111/jfd.12783.
13. Kawato S, Nozaki R, Kondo H, Hirono I. (2018) Draft genome sequence of Vibrio penaeicida strain TUMSAT-NU1, isolated from diseased shrimp in Japan. Genome Announc. 6(11). pii: e00153-18. doi: 10.1128/genomeA.00153-18.
14. Zhang K, Koiwai K, Kondo H, Hirono I. (2018) A novel white spot syndrome virus-induced gene (MjVIG1) from Marsupenaeus japonicus hemocytes. Fish Shellfish Immunol. 77:46-52. doi: 10.1016/j.fsi.2018.03.026
15. Angulo C, Alamillo E, Hirono I, Kondo H, Jirapongpairoj W, Perez-Urbiola JC, Reyes-Becerril M. (2018) Class B CpG-ODN2006 is highly associated with IgM and antimicrobial peptide gene expression through TLR9 pathway in yellowtail Seriola lalandi. Fish Shellfish Immunol. 77:71-82. doi: 10.1016/j.fsi.
16. Tandel GM, Kondo H, Hirono I. (2018) Gills specific type 2 crustin isoforms: Its molecular cloning and characterization from kuruma shrimp Marsupenaeus japonicus. Dev Comp Immunol. 85:25-30. doi: 10.1016/j.dci.2018.03.018.
17. Kawato S, Nozaki R, Kondo H, Hirono I. (2018) The complete mitochondrial genome sequence of the sakura shrimp, Sergia lucens (Crustacea, Decapoda, Sergestidae). Mitochondrial DNA Part B 3: 444-445. doi.org/10.1080/23802359.2018.1450671
18. Koiwai K, Kondo H, Hirono I. (2018) The immune functions of sessile hemocytes in three organs of kuruma shrimp Marsupenaeus japonicus differ from those of circulating hemocytes. Fish Shellfish Immunol. 78:109-113. doi: 10.1016/j.fsi. .2018.04.036.
19. Zhang K, Koiwai K, Kondo H, Hirono I. (2018) White spot syndrome virus (WSSV) suppresses penaeidin expression in Marsupenaeus japonicus hemocytes. Fish Shellfish Immunol. 78:233-237. doi: 10.1016/j.fsi.2018.04.045.
20. Koiwai K, Kodera T, Thawonsuwan J, Kawase M, Kondo H, Hirono I. (2018) Rapid diagnosis of three shrimp RNA viruses using RT-PCR-DNA chromatography. J Fish Dis. doi;: 10.1111/jfd.12821.
21. Sangsuriya P, Charoensapsri W, Sutthangkul J, Senapin S, Hirono I, Tassanakajon A, Amparyup P. (2018) Rapid diagnosis of three shrimp RNA viruses using RT-PCR-DNA chromatography. Dev Comp Immunol. 86:109-117. doi: 10.1016/j.dci.2018.05.005.
22. Tandel GM, Hipolito SG, Kondo H, Hirono I. (2018) Comparative sequence analysis of crustin isoform MjCRS7 and MjWFDC-like gene from kuruma shrimp Marsupenaeus japonicus shows variant of the WFDC domain. Infect Genet Evol. 64:139-148. doi: 10.1016/j.meegid.2018.06.008.
23. Pedrosa-Gerasmio IR, Tanaka T, Sumi A, Kondo H, Hirono I. (2018) Effects of 5-aminolevulinic acid on gene expression, immunity and ATP levels in Pacific white shrimp, Litopenaeus vannamei. Marine Biotechnol. doi: 10.1007/s10126-018-9852-2.

2017:
1. Thanasaksiri K, Hirono I, Kondo H. (2017) Molecular cloning and expression analysis of NOD-like receptor 5 in Japanese flounder (Paralichthys olivaceus) after injection with two different formalin-killed pathogenic bacteria and poly (I:C). Dev Comp Immunol. 67:481-484. doi: 10.1016/j.dci.2016.08.017.
2. Koiwai K, Alenton RR, Shiomi R, Nozaki R, Kondo H, Hirono I. (2017) Two hemocyte sub-populations of kuruma shrimp Marsupenaeus japonicas. Mol. Immunol. 85:1-8. doi: 10.1016/j.molimm.2017.01.024.
3. Takano T, Nakamura Y, Matsuyama T, Sakai T, Shigenobu Y, Sugaya T, Yasuike M, Fujiwara A, Kondo H, Hirono I, Fukuda Y, Nakayasu C. (2017) Complete Genome Sequence of Ichthyobacterium seriolicida JBKA-6T, Isolated from Yellowtail (Seriola quinqueradiata) Affected by Bacterial Hemolytic Jaundice. Genome Announc. 5(6). pii: e01574-16. doi: 10.1128/genomeA.01574-16.
4. Kannika K, Pisuttharachai D, Srisapoome P, Wongtavatchai J, Kondo H, Hirono I, Unajak S, Areechon N. (2017) Molecular serotyping, virulence gene profiling and pathogenicity of Streptococcus agalactiae isolated from tilapia farms in Thailand by multiplex PCR. J Appl Microbiol. 122:1497-1507, doi: 10.1111/jam.13447.
5. Alenton RR, Koiwai K, Miyaguchi K, Kondo H, Hirono I. (2017) Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif. Sci Rep. 7:45818. doi: 10.1038/srep4581
6. Reyes-Becerril M, Alamillo E, Trasvina A, Hirono I, Kondo H, Jirapongpairoj W, Ascencio-Valle F, Angulo C. (2017) In vivo and in vitro studies using larval and adult antigens from Neobenedenia melleni on immune response in yellowtail (Seriola lalandi). J Fish Dis. 40:1497-1509. doi:10.1111/jfd.12620.
7. Campa-Córdova AI, León-Gallo AF, Romero-Maldonado A, Ibarra-Serrano AC, Rosales-Mendoza S, Hirono I, Angulo C. (2017) Recombinant PirA-like toxin protects shrimp against challenge with Vibrio parahaemolyticus, the aetiological agent of acute hepatopancreatic necrosis disease. J Fish Dis. 40:1725-1729.doi: 10.1111/jfd.12625.
8. Hu Y, Yoshikawa T, Chung S, Hirono I, Kondo H. (2017) Identification of 2 novel type I IFN genes in Japanese flounder, Paralichthys olivaceus. Fish Shellfish Immunol. 67:7-10. doi: 10.1016/j.fsi.2017.05.054.
9. Kayansamruaj P, Dong HT, Hirono I, Kondo H, Senapin S, Rodkhum C. (2017) Comparative genome analysis of fish pathogen Flavobacterium columnare reveals extensive sequence diversity within the species. Infect Genet Evol. 54:7-17. doi: 10.1016/j.meegid.2017.06.012.
10. Rondón-Barragán I, Nozaki R, Hirono I, Kondo H. (2017) LAMP-1-chimeric DNA vaccines enhance the antibody response in Japanese flounder, Paralichthys olivaceus. Fish Shellfish Immunol. 67:546-553. doi: 10.1016/j.fsi.2017.06.045.
11. Jirapongpairoj W, Hirono I, Kondo H. (2017) Development and evaluation of polyclonal antisera for detection of the IgM heavy chain of multiple fish species. J Immunol Methods. 449:71-75. doi: 10.1016/j.jim.2017.06.011.
12. Khoa HV, Midorikawa Y, Uchino T, Nakai T, Kato G, Kondo H, Hirono I, Labaiden M, Direkbusarakom S, Sano M. (2017) Complete Genome Sequence of the Lytic Giant Bacteriophage pT24 Infecting Tenacibaculum spp., Isolated from a Shrimp Culture Pond. Genome Announc. 5(27). pii: e00081-17. doi: 10.1128/genomeA.00081-17.
13. Elbahnaswy S, Koiwai K, Zaki VH, Shaheen AA, Kondo H, Hirono I. (2017) A novel viral responsive protein (MjVRP) from Marsupenaeus japonicus haemocytes is involved in white spot syndrome virus infection. Fish Shellfish Immunol. 70: 638-647. doi: 10.1016/j.fsi.2017.09.045.

2016:
1. Lyu ZZ, Zhao BB, Koiwai K, Hirono I, Kondo H. (2016) Identification of endonuclease domain-containing 1 gene in Japanese flounder Paralichthys olivaceus. Fish Shellfish Immunol. 50:43-49. doi: 10.1016/j.fsi.2016.01.017.
2. Kaewkascholkul N, Somboonviwat K, Asakawa S, Hirono I, Tassanakajon A, Somboonwiwat K. (2016) Shrimp miRNAs regulate innate immune response against white spot syndrome virus infection. Dev Comp Immunol. 60:191-201. doi: 10.1016/j.dci.2016.03.002.
3. Jaree P, Senapin S, Hirono I, Lo CF, Tassanakajon A, Somboonwiwat K. (2016) WSV399, a viral tegument protein, interacts with the shrimp protein PmVRP15 to facilitate viral trafficking and assembly. Dev Comp Immunol. 59:177-185. doi: 10.1016/j.dci.2016.01.020.
4. Alenton RR, Kondo H, Hirono I, Maningas MB. (2016) Gene silencing of VP9 gene impairs WSSV infectivity on Macrobrachium rosenbergii. Virus Res. 214:65-70. doi: 10.1016/j.virusres.2016.01.013.
5. Reyes-Becerril M, Ascencio-Valle F, Hirono I, Kondo H, Jirapongpairoj W, Esteban MA, Alamillo E, Angulo C. (2016) TLR21's agonists in combination with Aeromonas antigens synergistically up-regulate functional TLR21 and cytokine gene expression in yellowtail leucocytes. Dev Comp Immunol. 61:107-115. doi: 10.1016/j.dci.2016.03.012.
6. Kaneko G, Shirakami H, Hirano Y, Oba M, Yoshinaga H, Khieokhajonkhet A, Nagasaka R, Kondo H, Hirono I, Ushio H. (2016) Diversity of Lipid Distribution in Fish Skeletal Muscle. Zoolog Sci. 33:170-178.
7. Areechon N, Kannika K, Hirono I, Kondo H, Unajak S. (2016) Draft Genome Sequences of Streptococcus agalactiae Serotype Ia and III Isolates from Tilapia Farms in Thailand. Genome Announc. 4(2). pii: e00122-16. doi: 10.1128/genomeA.00122-16.
8. Koiwai K, Alenton RR, Kondo H, Hirono I. (2016) Extracellular trap formation in kuruma shrimp (Marsupenaeus japonicus) hemocytes is coupled with c-type lysozyme. Fish Shellfish Immunol. 52:206-209. doi: 10.1016/j.fsi.2016.03.039.
9. Koiwai K, Tinwongger S, Nozaki R, Kondo H, Hirono I. (2016) Detection of acute hepatopancreatic necrosis disease strain of Vibrio parahaemolyticus using loop-mediated isothermal amplification. J Fish Dis. 39:603-606. doi: 10.1111/jfd.12387.
10. Reyes-Becerril M, Maldonado-García M, Guluarte C, León-Gallo A, Rosales-Mendoza S, Ascencio F, Hirono I, Angulo C. (2016) Evaluation of ToxA and Vibrio parahaemolyticus lysate on humoral immune response and immune-related genes in Pacific red snapper. Fish Shellfish Immunol. 56:310-21. doi: 10.1016/j.fsi.2016.07.014.
11. Mai HN, Nguyen HT, Koiwai K, Kondo H, Hirono I. (2016) Characterization of a Kunitz-type protease inhibitor (MjKuPI) reveals the involvement of MjKuPI positive hemocytes in the immune responses of kuruma shrimp Marsupenaeus japonicus. Dev Comp Immunol. 63:121-7. doi: 10.1016/j.dci.2016.05.022.
12. Tinwongger S, Nochiri Y, Thawonsuwan J, Nozaki R, Kondo H, Awasthi SP, Hinenoya A, Yamasaki S, Hirono I. (2016) Virulence of Acute Hepatopancreatic Necrosis Disease PirAB-like Relies on Secreted Proteins Not on Gene Copy Number. J Appl Microbiol. 121:1755-1765. doi: 10.1111/jam.13256.
13. Poolju Cn, Direkbusarakom S, Chotipuntu P, Hirono I, Wuthisuthimethavee S. (2016) Development of a TaqMan real-time RT-PCR assay for detection of covert mortality nodavirus (CMNV) in penaeid shrimp. Aquaculture, 464: 445-450. doi.org/10.1016/j.aquaculture.2016.06.044
14. Thanasaksiri K, Hirono I, Kondo H. (2016) Identification and expression analysis of suppressors of cytokine signaling (SOCS) of Japanese flounder Paralichthys olivaceus. Fish Shellfish Immunol. 58:145-152. doi: 10.1016/j.fsi.2016.09.018.
15. Kaneshige N, Jirapongpairoj W, Hirono I, Kondo H. (2016) Temperature-dependent regulation of gene expression in Japanese flounder Paralichthys olivaceus kidney after Edwardsiella tarda formalin-killed cells. Fish Shellfish Immunol. 59:298-304. doi: 10.1016/j.fsi.2016.10.048.

2015:
1. Kato G, Yamashita K, Kondo H, Hirono I. (2015) Protective efficacy and immune responses induced by a DNA vaccineencoding codon-optimized PPA1 against Photobacterium damselae subsp. piscicida in Japanese flounder. Vaccine, 33:1040-1045. doi: 10.1016/j.vaccine.2015.01.011.
2. Visetnan S, Supungul P, Hirono I, Tassanakajon A, Rimphanitchayakit V. (2015) Activation of PmRelish from Penaeus monodon by yellow head virus. Fish Shellfish Immunol. 42: 335-344. doi: 10.1016/j.fsi.2014.11.015.
3. Phetrungnapha A, Kondo H, Hirono I, Panyim S, Ongvarrasopone C. (2015) Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp. Fish Shellfish Immunol. 44:241-247. doi: 10.1016/j.fsi.2015.02.028.
4. Zhao B, Katagiri T, Kondo H, Hirono I. (2015) Comparative analysis of two types of CXCL8 from Japanese flounder (Paralichthys olivaceus). Dev Comp Immunol, 52: 37-47. doi: 10.1016/j.dci.2015.04.011.
5. Maralit BA, Komatsu M, Hipolito SG, Hirono I, Kondo H. (2015) Microarray analysis of immunity against WSSV in response to injection of non-specific long dsRNA in kuruma shrimp, Marsupenaeus japonicus. Mar Biotechnol, 17(4):493-501. doi: 10.1007/s10126-015-9637-9.
6. Thanasaksiri K, Hirono I, Kondo H. (2015) Temperature-dependent regulation of gene expression in poly (I:C)-treated Japanese flounder, Paralichthys olivaceus. Fish Shellfish Immunol. 45(2):835-40. doi: 10.1016/j.fsi.2015.05.036.
7. Reyes-Becerril M, Ascencio-Valle F, Alamillo E, Hirono I, Kondo H, Jirapongpairoj W, Angulo C. (2015) Molecular cloning and comparative responses of Toll-like receptor 22 following ligands stimulation and parasitic infection in yellowtail (Seriola lalandi). Fish Shellfish Immunol. 46(2):323-333, doi: 10.1016/j.fsi.2015.06.020.
8. Kondo H, Van PT, Dang LT, Hirono I. (2015) Draft Genome Sequence of Non-Vibrio parahaemolyticus Acute Hepatopancreatic Necrosis Disease Strain KC13.17.5, Isolated from Diseased Shrimp in Vietnam. Genome Announc. 2015 Sep 17;3(5). pii: e00978-15. doi: 10.1128/genomeA.00978-15.
9. Kayansamruaj P, Pirarat N, Kondo H, Hirono I, Rodkhum C. (2015) Genomic comparison between pathogenic Streptococcus agalactiae isolated from Nile tilapia in Thailand and fish-derived ST7 strains. Infect Genet Evol. 36:307-314. doi: 10.1016/j.meegid.2015.10.009.
10. Visetnan S, Supungul P, Tang S, Hirono I, Tassanakajon A, Rimphanitchayakit V. (2015) YHV-responsive gene expression under the influence of PmRelish regulation. Fish Shellfish Immunol. 2015 Oct 3;47(1):572-581. doi: 10.1016/j.fsi.2015.09.053.
11. Jirapongpairoj W, Kobayashi K, Fukuda Y, Takano T, Sakai T, Matsuyama T, Nakayasu C, Nozaki R, Hirono I, Kondo H. (2015) Development of consensus qPCR primers to detect cytokine genes in three amberjack species: Seriola quinqueradiata, S-lalandi and S-dumerili. Fish Sci, 81: 907-914.
12. Jariyapong P, Weerachatyanukul W, Direkbusarakom S, Hirono I, Wuthisuthimethavee, S, Chotwiwatthanakun C. (2015) Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA. Aquaculture, 446: 325-332.
13. Jariyapong P, Chotwiwatthanakun C, Direkbusarakom S, Hirono I, Wuthisuthimethavee S, Weerachatyanukul W. (2015) Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus. Aquaculture, 435: 86-91.
14. Takano T, Matsuyama T, Sakai T, Nakamura Y, Kamaishi T, Nakayasu C, Kondo H, Hirono I, Fukuda Y, Sorimachi M, Iida T. (2015) Ichthyobacterium seriolicida gen. nov., sp. nov., a member of the phylum Bacteroidetes, isolated from yellowtail fish (Seriola quinqueradiata) affected by bacterial hemolytic jaundice, and proposal of a new family, Ichthyobacteriaceae fam. nov. Int J Syst Evol Microbiol. doi: 10.1099/ijsem.0.000757.
15. Unajak S, Pholmanee N, Songtawee N, Srikulnath K, Srisapoome P, Kiataramkul A, Kondo H, Hirono I, Areechon N. (2015) Molecular characterization of Galectin-8 from Nile tilapia (Oreochromis niloticus Linn.) and its response to bacterial infection. Mol Immunol. 68(2 Pt C):585-96. doi: 10.1016/j.molimm.2015.09.012.

2014:
1. Unajak S, Sawatdichaikul O, Songtawee N, Rattanabunyong S, Tassnakajon A, Areechon N, Hirono I, Kondo H, Khunrae P, Rattanarojpong T, Choowongkomon K. (2014) Homology modeling and virtual screening for antagonists of protease from yellow head virus. J Mol Model. 20: 2116-2119.
2. Feroudj H, Matsumoto T, Kurosu Y, Kaneko G, Ushio H, Suzuki K, Kondo H, Hirono I, Nagashima Y, Akimoto S, Usui K, Kinoshita S, Asakawa S, Kodama M, Watabe S. (2014) DNA microarray analysis on gene candidates possibly related to tetrodotoxin accumulation in pufferfish. Toxicon, 77: 68-72.
3. Limtipsuntorn U, Haga Y, Kondo H, Hirono I, Satoh S. (2014) Microarray analysis of hepatic gene expression in juvenile Japanese flounder Paralichthys olivaceus fed diets supplemented with fish or vegetable oils. Mar. Biotechnol. 16: 88-102.
4. Kurokawa S, Kabayama J, Hwang SD, Nho SW, Hikima JI, Jung TS, Kondo H, Hirono I, Takeyama H, Mori T, Aoki T. (2014) Whole Genome analyses of marine fish pathogenic isolate, Mycobacterium sp. 012931. Mar Biotechnol. 16: 572-579
5. Kayansamruaj P, Pirarat N, Hirono I, Rodkhum C. (2014) Increasing of temperature induces pathogenicity of Streptococcus agalactiae and the up-regulation of inflammatory related genes in infected Nile tilapia (Oreochromis niloticus). Vet Microbiol, 172: 265-271.
6. Kayansamruaj P, Pirarat N, Katagiri T, Hirono I, Rodkhum C. (2014) Molecular characterization and virulence gene profiling of pathogenic Streptococcus agalactiae populations from tilapia (Oreochromis sp.) farms in Thailand. J Vet Diagn Invest. 26: 488-495.
7. Kondo H, Tinwongger S, Proespraiwong P, Mavichak R, Unajak S, Nozaki R, Hirono I. (2014) Draft Genome Sequences of Six Strains of Vibrio parahaemolyticus Isolated from Early Mortality Syndrome/Acute Hepatopancreatic Necrosis Disease Shrimp in Thailand. Genome Announc. 2(2). pii: e00221-14.
8. Hipolito SG, Shitara A, Kondo H, Hirono I. (2014) Role of Marsupenaeus japonicus crustin-like peptide against Vibrio penaeicida and white spot syndrome virus infection. Dev Comp Immunol. 46:461-469.
9. Hung MN, Shiomi R, Nozaki R, Kondo H, Hirono I. (2014) Identification of novel copper/zinc superoxide dismutase (Cu/ZnSOD) genes in kuruma shrimp Marsupenaeus japonicus. Fish Shellfish Immunol. 40: 472-477.
10. Thanasaksiri K, Sakai N, Yamashita H, Hirono I, Kondo H. (2014) Influence of temperature on Mx gene expression profiles and the protection of sevenband grouper, Epinephelus septemfasciatus, against red-spotted grouper nervous necrosis virus (RGNNV) infection after poly (I:C) injection. Fish Shellfish Immunol. 40: 441-445.
11. Zou GG, Nozaki R, Kondo H, Hirono I. (2014) Cloning and expression analysis of three novel CC chemokine genes from Japanese flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 40: 507-513.
12. Suraprasit S, Methatham T, Jaree P, Phiwsaiya K, Senapin S, Hirono I, Lo CF, Tassanakajon A, Somboonwiwat K. (2014) Anti-lipopolysaccharide factor isoform 3 from Penaeus monodon (ALFPm3) exhibits antiviral activity by interacting with WSSV structural proteins. Antiviral Res. 110C: 142-150.
13. Adamek M, Rakus KL, Brogden G, Matras M, Chyb J, Hirono I, Kondo H, Aoki T, Irnazarow I, Steinhagen D. (2014) Interaction between type I interferon and cyprinid herpesvirus 3 in two genetic lines of common carp Cyprinus carpio. Dis. Auat. Organ. 111:107-118.
14. Posiri P, Kondo H, Hirono I, Panyim S, Ongvarrasopone C. (2014) Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain. Aquaculture, 435: 480-487.
15. Kondo H, Kawana Y, Suzuki Y, Hirono I. (2014) Comprehensive gene expression profiling in Japanese flounder kidney after injection with two different formalin-killed pathogenic bacteria. Fish Shellfish Immunol. 41: 437-440.
16. Kayansamruaj P, Pirarat N, Kondo H, Hirono I, Rodkhum C. (2014) Draft Genome Sequences of Streptococcus agalactiae Strains Isolated from Nile Tilapia (Oreochromis niloticus) Farms in Thailand. Genome Announc. 2(6). pii: e01300-14.
17. Kato G, Kato K, Jirapongpairoj W, Kondo H, Hirono I. (2014) Development of DNA vaccines against Nocardia seriolae infection in fish. Fish Pathology, 49: 165-172.
18. Tinwongger S, Proespraiwong P, Thawonsuwan J, Sriwanayos P, Kongkumnerd J, Chaweepack T, Mavichak R, Unajak S, Nozaki R, Kondo H, Hirono I. (2014) Development of PCR diagnosis for shrimp acute hepatopancreatic necrosis disease (AHPND) strain of Vibrio parahaemolyticus. Fish Pathology, 49:159-164.

2013:
1. Ohtani M, Hikima JI, Jung TS, Kondo H, Hirono I, Takeyama H, Aoki T. (2013) Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library. Fish Shellfish Immunol, 34:724-8
2. Kaneko G, Yamada T, Han Y, Hirano Y, Khieokhajonkhet A, Shirakami H, Nagasaka R, Kondo H, Hirono I, Ushio H, Watabe S. (2013) Differences in lipid distribution and expression of peroxisome proliferator-activated receptor gamma and lipoprotein lipase genes in torafugu and red seabream. Gen Comp Endocrinol. 184:51-60
3. Kato G, Goto K, Akune I, Aoka S, Kondo H, Hirono I. (2013) CD4 and CD8 homologues in Japanese flounder, Paralichthys olivaceus: Differences in the expressions and localizations of CD4-1, CD4-2, CD8α and CD8β. Dev Comp Immunol. 39:293-301
4. Del Castillo CS, Hikima J, Jang HB, Nho SW, Jung TS, Wongtavatchai J, Kondo H, Hirono I, Takeyama H, Aoki T. (2013) Comparative sequence analysis of a multi-drug resistant plasmid from Aeromonas hydrophila. Antimicrob Agents Chemther, 57:120-9
5. Taechavasonyoo A, Kondo H, Nozaki R, Suzuki Y, Hirono I. (2013) Identification of novel interleukin 1 beta family genes in Japanese flounder Paralichthys olivaceus. Fish Shellfish Immunol, 34:393-6
6. Ohtani M, Hikima JI, Jung TS, Kondo H, Hirono I, Aoki T. (2013) Construction of an Artificially Randomized IgNAR Phage Display Library: Screening of Variable Regions that Bind to Hen Egg White Lysozyme. Mar Biotechnol, 15:56-62
7. Koyama T, Kondo H, Aoki T, Hirono I. (2013) Identification of two penelope-like elements with different structures and chromosome localization in kuruma shrimp genome. Mar. Biotechnol. 15:115-23
8. Kurokawa S, Kabayama J, Fukuyasu T, Hwang SD, Park CI, Park SB, Del Castillo CS, Hikima JI, Jung TS, Kondo H, Hirono I, Takeyama H, Aoki T. (2013) Bacterial classification of fish-pathogenic Mycobacterium species by multigene phylogenetic analyses and MALDI biotyper identification system. Mar Biotechnol, 15:340-8
9. Han Y, Kaneko G, Nagasaka R, Kondo H, Hirono I, Takahashi SI, Watabe S, Ushio H. (2013) Distribution of adipocyte-related cells in skeletal muscle of rainbow trout Oncorhynchus mykiss. Fish Sci, 79: 143-148.
10. Del Castillo CS, Jang HB, Hikima JI, Jung TS, Morii H, Hirono I, Kondo H, Kurosaka C, Aoki T. (2013) Comparative analysis and distribution of pP9014, a novel drug resistance IncP-1 plasmid from Photobacterium damselae subsp. piscicida. Int J Antimicrob Agents. 42:10-18.
11. Shitara A, Kondo H, Hirono I. (2013) New type of heat shock protein 70 homologue gene abounds in the genomic sequence of kuruma shrimp Marsupenaeus japonicus. Fish Sci, 79: 397-405.
12. Kurokawa S, Kabayama J, Hwang SD, Nho SW, Hikima JI, Jung TS, Sakai M, Kondo H, Hirono I, Takeyama H, Aoki T. (2013) Comparative genome analysis of fish and human isolates of Mycobacterium marinum. Mar Biotechnol, 15: 596-605.
13. Kurokawa S, Kabayama J, Nho SW, Hwang SD, Hikima JI, Jung TS, Kondo H, Hirono I, Takeyama H, Aoki T. (2013) Whole-Genome Sequence of Fish-Pathogenic Mycobacterium sp. Strain 012931, Isolated from Yellowtail (Seriola quinqueradiata). Genome Announc. 1(4). e00534-13.
14. Kitagima RE, Haga Y, Hirono I, Satoh S. (2013) Studies on transcription initiated by cauliflower mosaic virus 35S promoter from transgenic crops using fish cell line (HINAE, YO-K, RTG-2) and rainbow trout Oncothynchus mykiss. Aquacult Nutr. 19:122-134.
15. Taechavasonyoo A, Hirono I, Kondo H. (2013) The immune-adjuvant effect of Japanese flounder Paralichthys olivaceus IL-1β. Dev Comp Immunol. 41: 564-8.
16. Nho SW, Hikima J, Park SB, Jang HB, Cha IS, Yasuike M, Nakamura Y, Fujiwara A, Sano M, Kanai K, Kondo H, Hirono I, Takeyama H, Aoki T, Jung TS. (2013) Comparative Genomic Characterization of Three Streptococcus parauberis Strains in Fish Pathogen, as Assessed by Wide-Genome Analyses. PLoS One. 8(11):e80395.

2012:
1. Ohtani M, Hikima JI, Hwang SD, Morita T, Suzuki Y, Kato G, Kondo H, Hirono I, Jung TS, Aoki T. (2012) Transcriptional regulation of type I interferon gene expression by interferon regulatory factor-3 in Japanese flounder, Paralichthys olivaceus. Dev Comp Immunol. 36: 697-706.
2. Kato G, Kondo H, Aoki T, Hirono I. (2012) A novel immune-related gene, microtubule aggregate protein homologue, is up-regulated during IFN-γ-related immune responses in Japanese flounder, Paralichthys olivaceus. Dev Comp Immunol. 36:349-358.
3. Encarnacion AB, Fagutao F, Orapint Jintasataporn, Wanchai Worawattanamateekul, Hirono I, Ohshima T. (2012) Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei. Food Res Internat. 45: 232-237.
4. Park SB, Hikima JI, Suzuki Y, Ohtani M, Nho SW, Cha IS, Jang HB, Kondo H, Hirono I, Aoki T, Jung TS. (2012) Molecular cloning and functional analysis of nucleotide-binding oligomerization domain 1 (NOD1) in olive flounder, Paralichthys olivaceus. Dev Comp Immunol. 36: 680-687
5. Rakus KL, Irnazarow I, Adamek M, Palmeira L, Kawana Y, Hirono I, Kondo H, Matras M, Steinhagen D, Flasz B, Brogden G, Vanderplasschen A, Aoki T. (2012) Gene expression analysis of common carp (Cyprinus carpio L.) lines during Cyprinid herpesvirus 3 infection yields insights into differential immune responses. Dev Comp Immunol. 37: 65-76.
6. Hwang SD, Ohtani M, Hikima JI, Jung TS, Kondo H, Hirono I, Aoki T. (2012) Molecular cloning and characterization of Toll-like receptor 3 in Japanese flounder, Paralichthys olivaceus. Dev Comp Immunol. 37: 87-96.
7. Fagutao FF, Maningas MB, Kondo H, Aoki T, Hirono I. (2012) Transglutaminase regulates immune-related genes in shrimp. Fish Shellfish Immunol. 32: 711-715.
8. Kato G, Kondo H, Aoki T, Hirono I. (2012) Mycobacterium bovis BCG vaccine induces non-specific immune responses in Japanese flounder against Nocardia seriolae. Fish Shellfish Immunol. 33:243-250.
9. Hwang SD, Midorikawa N, Punnarak P, Kikuchi Y, Kondo H, Hirono I, Aoki T. (2012) Inhibition of hirame rhabdovirus growth by RNA aptamers. J Fish Dis. 35: 927-934.
10. Punnarak P, Santos MD, Hwang SD, Kondo H, Hirono I, Kikuchi Y, Aoki T. (2012) RNA Aptamers Inhibit the Growth of the Fish Pathogen Viral Hemorrhagic Septicemia Virus (VHSV). Mar Biotechnol, 14:752-61
11. Kondo H, Suda S, Kawana Y, Hirono I, Nagasaka R, Kaneko G, Ushio H, Watabe S. (2012) Effects of feed restriction on the gene expression profiles of adiponectin and energy metabolism genes in rainbow trout Oncorhynchus mykiss muscle. Fish Sci, 78: 1205-1211.
12. Maningas MB, Kondo H, Hirono I. (2012) Molecular mechanisms of the shrimp clotting system. Fish Shellfish Immunol, (review) 34:968-72.

著書

いろいろ

氏名

廣野育生 

読み

HIRONO, Ikuo

役職

教授

所属部門

海洋生物資源学部門

研究室紹介

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